Topoisomerase II alpha 抗体 (Middle Region)
Our Local Distributor
北京 101111
Quick Overview for Topoisomerase II alpha 抗体 (Middle Region) (ABIN8047555)
抗原
See all Topoisomerase II alpha (TOP2A) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
-
-
抗原表位
- Middle Region
-
原理
- Anti-Topoisomerase II alpha/TOP2A Antibody
-
特异性
- No cross reactivity with other proteins.
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
预测反应
- identical to the related mouse,rat sequences.
-
产品特性
- Anti-Topoisomerase II alpha/TOP2A Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Topoisomerase II alpha/TOP2A, identical to the related mouse and rat sequences.
-
亚型
- IgG
-
-
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1435-1531) antibody
TOP2A 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E2 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) antibodyTOP2A 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1352-1493) antibodyTOP2A 适用: 人, 小鼠 WB, IHC, IF 宿主: 小鼠 Monoclonal TOP2A-1361 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1100-1530) antibodyTOP2A 适用: 人, 大鼠 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 6C12G12 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) antibodyKD Validated TOP2A 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 24GB1915 unconjugated Recombinant Antibody
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1100-1530) antibodyTOP2A 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6D4B2 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (Internal Region) antibodyTOP2A 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1167-1196), (C-Term) antibodyTOP2A 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB24346 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (Middle Region) antibodyTOP2A 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1352-1493) antibodyTOP2A 适用: 人 IHC 宿主: 兔 Monoclonal 802R unconjugated Recombinant Antibody
-
-
应用备注
- Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
-
-
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
-
别名
- TOP2A
-
背景
-
Background: The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 Molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1 % of the nuclear extract1. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II2.
Gene Full Name: DNA topoisomerase II alpha
-
分子量
- 174 kDa
-
基因ID
- 7153
-
UniProt
- P11388
-
途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
抗原
-
