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PKC alpha 抗体 (AA 153-342)

The 兔 多克隆 anti-PKC alpha antibody is suitable to detect PKC alpha in samples from 人, 小鼠 和 大鼠. It has been validated for WB 和 IHC.
产品编号 ABIN8027409
发货至: 中国
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Quick Overview for PKC alpha 抗体 (AA 153-342) (ABIN8027409)

抗原

See all PKC alpha (PKCa) 抗体
PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This PKC alpha antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)

质量等级

Carrier-free
  • 抗原表位

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    AA 153-342

    原理

    Anti-PKC alpha/PRKCA Antibody

    特异性

    No cross reactivity with other proteins.

    交叉反应 (详细)

    No cross-reactivity with other proteins

    预测反应

    Human PKC alpha shares 97%,99% amino acid (aa) sequence identity with mouse,rat PKC alpha,respectively.

    产品特性

    Anti-PKC alpha/PRKCA Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human PKC alpha recombinant protein (Position: M153-L342). Human PKC alpha shares 97% and 99% amino acid (aa) sequence identity with mouse and rat PKC alpha, respectively.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

    别名

    PRKCA

    背景

    Background: Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is thought to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PRKCA1 regulates cardiac contractility and propensity toward heart failure.

    Gene Full Name: protein kinase C alpha

    Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

    分子量

    77 kDa

    基因ID

    5578

    UniProt

    P17252

    途径

    WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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