我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

PKC alpha 抗体 (C-Term)

Name: PKC alpha 抗体 (C-Term)
SKU: ABIN391001
Availability: OutOfStock

PKCa 适用: 人 WB, FACS, IHC (p), IF 宿主: 兔 Polyclonal RB01012 unconjugated

Western blot analysis of anti-PKCalpha Pab (ABIN391001 and ABIN2841177) in placenta lysate.

PKC alpha Antibody (C-term) (ABIN391001 and ABIN2841177) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).

FACS

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

Confocal immunofluorescent analysis of PKC alpha Antibody (C-term) (ABIN391001 and ABIN2841177) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

4 images

(1 reference)

产品编号 ABIN391001

发货至: 中国

Datasheet as PDF

抗原 See all PKC alpha (PKCa) 抗体

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
抗原表位
24

20

18

12

10

10

7

7

7

6

6

5

5

5

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 299-329, C-Term
适用
177

90

68

6

5

5

3

2

2

1

1

1

1
人
宿主
173

12

1
兔
Compatible Secondaries
克隆类型
139

46
多克隆
标记
95

15

13

6

6

6

6

6

6

6

6

6

2

2

1

1

1

1

1
This PKC alpha antibody is un-conjugated
应用范围
127

71

53

32

22

19

16

15

14

8

5

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

预测反应

M, Rb, Rat

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This PKC alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-329 amino acids from the C-terminal region of human PKC alpha.

克隆位点

RB01012

亚型

Ig Fraction

Top Product

Discover our top product PKCa Primary Antibody
anti-Protein Kinase C, alpha (PKCa) (AA 563-672) antibody
PKCa 适用: 人 WB, ELISA, IHC (p), IF, PLA 宿主: 小鼠 Monoclonal 2F11 unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IP, IF, ICC 宿主: 兔 Monoclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329) antibody
PKCa 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠 WB, FACS, IHC (p), IF (cc) 宿主: 兔 Monoclonal 4B3 unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody
PKCa 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (N-Term) antibody
PKCa 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Jaafar, Zeiller, Pirola, Di Grazia, Naro, Vidal, Lefai, Némoz: "Phospholipase D regulates myogenic differentiation through the activation of both mTORC1 and mTORC2 complexes." in: The Journal of biological chemistry, Vol. 286, Issue 25, pp. 22609-21, (2011) (PubMed).
抗原

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

别名

PKC alpha (PKCa 产品)

别名

pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody

背景

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC alpha is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

分子量

76750

基因ID

5578

NCBI登录号

NP_002728

UniProt

P17252

途径

WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling