beta Actin 抗体 (N-Term)
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Quick Overview for beta Actin 抗体 (N-Term) (ABIN7965703)
抗原
See all beta Actin (ACTB) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- Anti-beta Actin/ACTB Antibody
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特异性
- No cross reactivity with other proteins.
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交叉反应 （详细）
- No cross reactivity with other proteins
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预测反应
- identical to the related rat,mouse sequences.
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产品特性
- Anti-beta Actin/ACTB Antibody. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken, Zebrafish.
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纯化方法
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人, 小鼠, 大鼠, Pig, 兔, Cow WB, ELISA, IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) antibodyACTB 适用: 人 WB, IF, IP 宿主: 小鼠 Monoclonal unconjugated
anti-Actin, beta (ACTB) (AA 2-16) antibodyACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) antibodyACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated
anti-Actin, beta (ACTB) (AA 1-25) antibodyACTB 适用: 人, 小鼠, 大鼠, Pig, Cow WB, IHC (p) 宿主: 小鼠 Monoclonal 1A2 unconjugated
anti-Actin, beta (ACTB) (AA 1-375) antibodyACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated
anti-Actin, beta (ACTB) (N-Term) antibodyKO Validated ACTB 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 1-375) antibodyACTB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 350-375) antibodyACTB 适用: 人, 小鼠, Leopard Frog WB, ELISA, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) antibodyACTB 适用: 人, 小鼠, 小鸡, Pig, 兔, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat, Chicken, Zebrafish
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Sodium azide, Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- beta Actin (ACTB) (Actin, beta (ACTB))
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别名
- ACTB
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背景
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Background: beta-Actin (ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al. (1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al. (2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al. (2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.
Gene Full Name: actin beta
Sequence Similarities: Belongs to the actin family.
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分子量
- 42 kDa
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基因ID
- 60
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UniProt
- P60709
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途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
抗原
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