beta Actin 抗体 (N-Term) (HRP)
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Quick Overview for beta Actin 抗体 (N-Term) (HRP) (ABIN7965699)
抗原
See all beta Actin (ACTB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Anti-beta Actin/ACTB Antibody HRP Conjugated
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特异性
- No cross reactivity with other proteins.
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交叉反应 (详细)
- No cross reactivity with other proteins
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预测反应
- identical to the related rat,mouse sequences.
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纯化方法
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.
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亚型
- IgG
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应用备注
- Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.
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储存条件
- -20 °C
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储存方法
- At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.
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有效期
- 12 months
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- beta Actin (ACTB) (Actin, beta (ACTB))
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别名
- ACTB
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背景
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Background: beta-Actin (ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al. (1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al. (2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al. (2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.
Gene Full Name: actin beta
Sequence Similarities: Belongs to the actin family.
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基因ID
- 60
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UniProt
- P60709
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途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
抗原
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