PRAS40 抗体 (pThr246)
Quick Overview for PRAS40 抗体 (pThr246) (ABIN744953)
抗原
See all PRAS40 (AKT1S1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr246
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特异性
- This phosphorylation site is homologous to Thr247 in Mouse and Rat.
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预测反应
- Human,Mouse,Rat,Dog,Cow,Horse
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic phosphopeptide derived from human PRAS40 around the phosphorylation site of Thr246
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亚型
- IgG
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anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 92-276) antibody
AKT1S1 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 6H1C6 unconjugated
anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 152-276) antibodyAKT1S1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 92-276) antibodyAKT1S1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4A11G7 unconjugated
anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 52-101) antibodyAKT1S1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 132-256) antibodyAKT1S1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) antibodyAKT1S1 适用: 人 WB, IHC, IHC (p), IP 宿主: 小鼠 Monoclonal unconjugated
anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 227-256) antibodyAKT1S1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) antibodyAKT1S1 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal 1997CT730-74-73 unconjugated
anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (pThr356) antibodyAKT1S1 适用: Drosophila melanogaster WB 宿主: 兔 Polyclonal unconjugated
anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 202-251) antibodyAKT1S1 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, Pig, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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- PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
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别名
- PRAS40
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背景
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Synonyms: Lobe, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate, AKT1S1
Background: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
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基因ID
- 84335
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UniProt
- Q96B36
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect
抗原
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