CEA 抗体
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北京 101111
Quick Overview for CEA 抗体 (ABIN7884238)
抗原
See all CEA 抗体片段
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Carcinoembryonic antigen (CEA) F(ab')2 Mouse Monoclonal Antibody (C234)
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特异性
- This antibody is CEA specific. It does not recognize NCA, neither in enzyme linked immunosorbent assay (ELISA) not on Western blots of crude tissue extracts. This antibody binds to CEA producing human adenocarcinoma cells (e.g. LS174T,WiDr) but does not bind to CEA non-secreting human adenocarcinoma cells (Colo 320DM). It does not immunostain cryocuts of any normal human tissues tested (artery, heart muscle, kidney, large bowel, liver, lung, skeletal muscle, skin, small gut, spleen, testes or vein) neither does it react with any antigens on human blood cells when separated by fluoresceine actuated cell sorter (FACS). Immunohistochemical analysis of cancerous tissues revealed that the antibody bound selectively adenocarcinoma and not carcinoid tumor of the colon, as well as tumor- associated CEA in breast, gastric and lung neoplasms, while there was no immunohistochemical evidence of cross-reactivity with NCA. A remarkable feature of this antibody is its ability to distinguish immunohistochemically between cancer and residual normal tissue within the same organ.
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纯化方法
- Purified
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免疫原
- Physically modified CEA
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亚型
- IgG kappa
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anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 448-572) antibody
CEA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 WB, IHC (p), ELISA, IF (p) 宿主: 小鼠 Monoclonal B5 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 315-365) antibodyCEA 适用: 人 WB, IHC (p), ELISA, IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1C7 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 3G12 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 400-429) antibodyCEA 适用: 人 IHC, WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 IHC (p), ELISA, IF (p), IF (cc) 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) (full length) antibodyCEA 适用: 人 IHC 宿主: 兔 Monoclonal 465R unconjugated Recombinant Antibody
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 400-429) antibodyCEA 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB19418 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 IHC, Lbl 宿主: 小鼠 Monoclonal A5B7 unconjugated Recombinant Antibody
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with deionized water. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized Protein A purified in PBS pH 7.4 (F(ab'2))
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- CEA (Carcinoembryonic Antigen Gene Family (CEA))
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别名
- Carcinoembryonic antigen (CEA)
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背景
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CEA
Carcinoembronic antigen (CEA), an oncodevelopmental tumor marker is a fairly typical, high-molecular weight, cell surface glycoprotein over-expressed in adenocarcinomas of entero-dermally-derived digestive system epithelia and in fetal colon. The majority of antibodies induced against CEA would cross-react with a variety of proteins present in normal tissues such as non-specific cross-reacting antigen (NCA). Highly specific anti-CEA mouse monoclonal antibody was produced as a result of a unique imunization protocol.
抗原
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