SUMO1 抗体
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北京 101111
Quick Overview for SUMO1 抗体 (ABIN7882286)
抗原
See all SUMO1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-SUMO-1 Antibody
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human protein was used as the immunogen for this anti-SUMO-1 antibody.
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亚型
- IgG1, kappa
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应用备注
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The optimal dilution of the anti-SUMO-1 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-SUMO-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))
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别名
- SUMO-1
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背景
- This mAb is specific to SUMO-1 and shows no cross-reaction with either SUMO2 or SUMO3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, SUMO2 and SUMO3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized is precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO1 protein localizes to the nuclear membrane.
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UniProt
- P63165
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途径
- M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway
抗原
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