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MUC5AC 抗体

This 小鼠 单克隆 antibody specifically detects MUC5AC in IHC (p). It exhibits reactivity toward 人.
产品编号 ABIN7881678
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for MUC5AC 抗体 (ABIN7881678)

抗原

See all MUC5AC 抗体
MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This MUC5AC antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPM297
  • 原理

    Mucin-5AC Antibody

    纯化方法

    Protein G affinity chromatography

    免疫原

    An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this Mucin-5AC antibody.

    亚型

    IgG1, kappa
  • 应用备注

    The optimal dilution of the Mucin-5AC antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Mucin-5AC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

    别名

    Mucin-5AC

    背景

    This mAb recognizes the peptide core of gastric mucin M1 (>1,000 kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

    UniProt

    P98088
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