MUC5AC 抗体
Our Local Distributor
北京 101111
Quick Overview for MUC5AC 抗体 (ABIN7881678)
抗原
See all MUC5AC 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Mucin-5AC Antibody
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this Mucin-5AC antibody.
-
亚型
- IgG1, kappa
-
-
-
-
应用备注
-
The optimal dilution of the Mucin-5AC antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store the Mucin-5AC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
-
-
- MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))
-
别名
- Mucin-5AC
-
背景
- This mAb recognizes the peptide core of gastric mucin M1 (>1,000 kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
-
UniProt
- P98088
抗原
-