Recombinant DOT1L 抗体
Our Local Distributor
北京 101111
Quick Overview for Recombinant DOT1L 抗体 (ABIN7880643)
抗原
See all DOT1L 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- DOT1L Antibody / KMT4
-
纯化方法
- Affinity purified
-
免疫原
- A synthetic peptide specific to Disruptor of telomeric silencing 1 like protein was used as the immunogen for the DOT1L antibody.
-
亚型
- IgG
-
-
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (AA 3-108) antibody
DOT1L 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6A6 unconjugated
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (AA 331-484) antibodyDOT1L 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (AA 1390-1420), (C-Term) antibodyDOT1L 适用: 人 IHC (p) 宿主: 兔 Polyclonal RB2773 unconjugated
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (AA 1000-1050) antibodyDOT1L 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (AA 1000-1050) antibodyDOT1L 适用: 人 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (AA 51-150) antibodyDOT1L 适用: 人, 小鼠, 大鼠, Green Monkey WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (C-Term) antibodyDOT1L 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (AA 3-108) antibodyDOT1L 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (C-Term) antibodyDOT1L 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) antibodyDOT1L 适用: 人 WB, ELISA 宿主: 兔 Monoclonal 3B6 unconjugated Recombinant Antibody
-
-
应用备注
- Optimal dilution of the DOT1L antibody should be determined by the researcher.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Antibody in PBS with 0.02 % sodium azide, 50 % glycerol and 0.4-0.5 mg/mL BSA
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store the DOT1L antibody at -20oC.
-
-
- DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))
-
别名
- DOT1L
-
背景
-
DOT1L antibody is a widely used reagent for studying epigenetic control, chromatin remodeling, and transcriptional regulation. The encoded protein, KMT4 (also known as disruptor of telomeric silencing 1 like), is a histone methyltransferase that specifically methylates histone H3 on lysine 79. This unique modification, located within the globular domain of histone H3, is strongly linked to transcriptional activation, DNA repair, and maintenance of genomic stability. Unlike most lysine methyltransferases that contain a SET domain, DOT1L is structurally distinct, highlighting its specialized role in epigenetic regulation.
KMT4 functions by altering chromatin accessibility and creating binding sites for regulatory proteins that control transcription and DNA damage response. During development, DOT1L plays critical roles in embryogenesis, hematopoiesis, and differentiation. Loss of DOT1L disrupts stem cell renewal and lineage commitment, demonstrating its essential role in developmental biology. Its activity is also important for maintaining telomeric silencing and ensuring proper chromosomal function.
Aberrant DOT1L activity has been strongly implicated in cancer. In mixed lineage leukemia (MLL) rearranged leukemias, DOT1L is aberrantly recruited to oncogenic transcriptional complexes, leading to inappropriate H3K79 methylation and activation of leukemia promoting genes. This discovery has made DOT1L a therapeutic target, and small molecule inhibitors of DOT1L are in clinical trials for treatment of MLL associated leukemias. Beyond leukemia, dysregulated DOT1L activity has been observed in solid tumors, cardiovascular disease, and viral infection, underscoring its wide biological significance.
At the molecular level, DOT1L associates with transcriptional elongation complexes and other chromatin modifiers, linking histone modification with gene expression control. Structural studies show that DOT1L interacts directly with the nucleosome core, uniquely positioning it to catalyze methylation of H3K79. Through these mechanisms, DOT1L serves as an integrator of epigenetic signaling and transcriptional activity, shaping the cellular transcriptome in response to developmental and environmental signals.
The DOT1L antibody is commonly used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect expression levels, nuclear localization, and disease associated changes. These applications are valuable for exploring transcriptional regulation, epigenetic therapy, and cancer biology. For researchers studying chromatin structure, RNA polymerase II elongation, or targeted therapeutics, the DOT1L antibody provides a reliable and specific detection tool. NSJ Bioreagents offers validated antibodies designed to ensure accuracy and reproducibility in advanced molecular research. -
UniProt
- Q8TEK3
抗原
-
