Crossover junction endonuclease EME1 (EME1) 抗体
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Quick Overview for Crossover junction endonuclease EME1 (EME1) 抗体 (ABIN7880514)
抗原
See all Crossover junction endonuclease EME1 (EME1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- EME1 Antibody
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纯化方法
- Protein G affinity chromatography
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免疫原
- His-tagged recombinant Eme1 of human origin was used as the immunogen for the EME1 antibody.
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亚型
- IgG1, kappa
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anti-Crossover junction endonuclease EME1 (EME1) (AA 1-583) antibody
EME1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (AA 520-561), (C-Term) antibodyEME1 适用: 人, 大鼠 WB, IHC, ICC, IHC (fro), FACS 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (Internal Region) antibodyEME1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (AA 1-583) antibodyEME1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (AA 1-320) antibodyEME1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (C-Term) antibodyEME1 适用: 人, 小鼠, Pig, 犬, 豚鼠, 兔, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (N-Term) antibodyEME1 适用: 人, 小鼠, Pig, Cow, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (Internal Region) antibodyEME1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the EME1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the EME1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Crossover junction endonuclease EME1 (EME1)
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别名
- EME1
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背景
- Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3 DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.
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UniProt
- Q96AY2
抗原
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