Crossover junction endonuclease EME1 (EME1) 抗体
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- 抗原 See all Crossover junction endonuclease EME1 (EME1) 抗体
- Crossover junction endonuclease EME1 (EME1)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
- 特异性
- Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3' DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.
- 交叉反应 （详细）
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- His-tagged recombinant Eme1 of human origin
- 亚型
- IgG1
- Top Product
- Discover our top product EME1 Primary Antibody
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anti-Crossover junction endonuclease EME1 (EME1) (AA 1-583) antibody
EME1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (AA 1-583) antibodyEME1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (Internal Region) antibodyEME1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (AA 520-561), (C-Term) antibodyEME1 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) antibodyEME1 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (N-Term) antibodyEME1 适用: 人, 小鼠, Pig, Cow, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (C-Term) antibodyEME1 适用: 人, 小鼠, Pig, 犬, 豚鼠, 兔, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (AA 1-320) antibodyEME1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (Internal Region) antibodyEME1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HEK293T or U-87 MG cells. Human tonsil.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Crossover junction endonuclease EME1 (EME1)
- 别名
- EME1 (EME1 产品)
- 别名
- 6820428D13 antibody, essential meiotic structure-specific endonuclease 1 antibody, Eme1 antibody
- 背景
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Crossover junction endonuclease EME1, Essential Meiotic Endonuclease 1 Homolog 1/2, hMMS4, MMS4L, SLX2A,EME1
Cellular localisation: Nucleus - 分子量
- 65kDa
- 基因ID
- 146956, 514330
- UniProt
- Q96AY2
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