CEA 抗体
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北京 101111
Quick Overview for CEA 抗体 (ABIN7880185)
抗原
See all CEA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- CEA Antibody / CEACAM5
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纯化方法
- Protein G affinity chromatography
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免疫原
- Purified human protein was used as the immunogen for the CEA antibody.
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亚型
- IgG1, kappa
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应用备注
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Optimal dilution of the CEA antibody to be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CEA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- CEA (Carcinoembryonic Antigen Gene Family (CEA))
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别名
- CEA
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背景
- This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This mAb reacts with nonspecific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leukocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
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UniProt
- P06731
抗原
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