Recombinant p130 抗体 (pThr986)
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Quick Overview for Recombinant p130 抗体 (pThr986) (ABIN7879813)
抗原
See all p130 (RBL2) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pThr986
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原理
- Phospho-RBL2 (Thr986) Antibody / Retinoblastoma-like protein 2
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Phospho-p130(T986) was used as the immunogen for the Phospho-RBL2 (Thr986) antibody.
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亚型
- IgG
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anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 939-1139) antibody
RBL2 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2C8A8 unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 165-194), (N-Term) antibodyRBL2 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB31021 unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) antibodyRBL2 适用: 大鼠, 小鼠 IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 100-200) antibodyRBL2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) antibodyRBL2 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 416-515) antibodyRBL2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 416-515) antibodyRBL2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4D7 unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (C-Term) antibodyRBL2 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 417-616) antibodyRBL2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 175-189) antibodyRBL2 适用: 人 WB, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the Phospho-RBL2 (Thr986) antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the Phospho-RBL2 (Thr986) antibody at -20oC.
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- p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))
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别名
- RBL2
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背景
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Phospho-RBL2 (Thr986) antibody detects retinoblastoma-like protein 2 phosphorylated at threonine 986, a modification regulating its function in the cell cycle. Also known as p130, RBL2 belongs to the retinoblastoma family of tumor suppressors, which includes RB1 and RBL1. RBL2 represses E2F transcription factors, preventing premature entry into S phase. Phosphorylation of RBL2 at Thr986 disrupts its repressive activity, allowing transcription of genes required for DNA replication and cell cycle progression.
Research using Phospho-RBL2 (Thr986) antibody demonstrates its role in proliferative control and cancer biology. Hyperphosphorylation of RBL2 leads to deregulated E2F activity, promoting uncontrolled growth and oncogenesis. This phosphorylation event is mediated by cyclin-dependent kinases, linking RBL2 regulation to signaling pathways frequently altered in tumors. Thr986 phosphorylation is therefore a valuable marker of cell cycle status and a potential therapeutic target.
Beyond cancer, phosphorylation of RBL2 influences cellular senescence and differentiation. Studies suggest that RBL2 integrates signals from growth factors and stress pathways to determine cell fate decisions. Tracking phospho-RBL2 helps clarify how cells transition between quiescence, proliferation, and terminal differentiation. This information is useful in both basic biology and translational research aiming to control growth in disease contexts.
Antibodies against phospho-RBL2 (Thr986) are validated for western blot, immunohistochemistry, and immunofluorescence. These reagents allow selective detection of the phosphorylated form, ensuring accurate assessment of cell cycle-dependent regulation. Clone-based antibodies provide specificity required to differentiate between phosphorylated and unmodified RBL2.
NSJ Bioreagents provides this Phospho-RBL2 (Thr986) antibody for cancer biology, cell cycle, and signaling research. -
UniProt
- Q08999
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
抗原
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