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Recombinant PKC alpha 抗体 (pThr497)

Name: Recombinant PKC alpha 抗体 (pThr497)
SKU: ABIN7879798
Availability: OutOfStock

The 兔单克隆 anti-PKC alpha antibody (Clone 32P00) (ABIN7879798) specifically detects PKC alpha in WB 和 IHC. The antibody is reactive with 人, 小鼠和大鼠 samples.

产品编号 ABIN7879798

发货至: 中国

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Quick Overview for Recombinant PKC alpha 抗体 (pThr497) (ABIN7879798)

抗原

See all PKC alpha (PKCa) 抗体

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

抗体类型

Recombinant Antibody

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

单克隆

标记

This PKC alpha antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

32P00

Discover our top product PKCa Primary Antibody

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pThr497

原理

Phospho-PKC alpha (Thr497) Antibody / Protein kinase C alpha

纯化方法

Affinity-chromatography

免疫原

A synthesized peptide derived from human Phospho-PKC alpha (T497) was used as the immunogen for the Phospho-PKC alpha (Thr497) antibody.

亚型

IgG
anti-Protein Kinase C, alpha (PKCa) (AA 563-672) antibody
PKCa 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 2F11 unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
KD Validated PKCa 适用: 人 WB, ICC, FACS, IF (p) 宿主: 兔 Monoclonal 23GB1690 unconjugated Recombinant Antibody

anti-Protein Kinase C, alpha (PKCa) (pThr497) antibody
KD Validated PKCa 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 23GB4955 unconjugated Recombinant Antibody

anti-Protein Kinase C, alpha (PKCa) antibody
KD Validated PKCa 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 24GB4475 unconjugated Recombinant Antibody

anti-Protein Kinase C, alpha (PKCa) (AA 153-342) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329), (C-Term) antibody
PKCa 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB01012 unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329) antibody
PKCa 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠 WB, IHC (p), FACS, IF (cc) 宿主: 兔 Monoclonal 4B3 unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (pSer657) antibody
KD Validated PKCa 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB1055 unconjugated Recombinant Antibody

应用备注

Optimal dilution of the Phospho-PKC alpha (Thr497) antibody should be determined by the researcher.

限制

仅限研究用
状态

Liquid

缓冲液

Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store the Phospho-PKC alpha (Thr497) antibody at -20oC.
抗原

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

别名

PKC alpha

背景

Phospho-PKC alpha Thr497 antibody recognizes protein kinase C alpha phosphorylated at threonine 497, an activation site essential for catalytic function. PKC alpha is a member of the conventional protein kinase C family that functions as a serine threonine kinase. It is activated by calcium, diacylglycerol, and phospholipids, and it regulates processes including cell proliferation, differentiation, apoptosis, and migration. Phosphorylation at Thr497 stabilizes the kinase in an active conformation and promotes downstream substrate phosphorylation.

Phospho-PKC alpha Thr497 antibody is widely applied in signaling and phosphorylation research. By distinguishing the phosphorylated active form from inactive PKC alpha, it enables precise monitoring of pathway activation. This is particularly relevant in studies of cancer, cardiovascular disease, and neuronal signaling where PKC alpha activity is tightly regulated.

The antibody is validated for use in western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho PKC alpha Thr497 antibody detects phosphorylated isoforms and allows quantification of activation under various stimuli. In immunohistochemistry, it highlights phosphorylation dependent localization within tissues, while immunofluorescence provides single cell resolution of active PKC alpha.

PKC alpha has been implicated in cancer progression, where dysregulated activation contributes to survival, proliferation, and metastasis. Monitoring Thr497 phosphorylation with phospho specific antibodies provides mechanistic insight into tumor biology and therapeutic targeting. In cardiovascular research, PKC alpha activity is linked to hypertrophy, contractility, and vascular remodeling. In the nervous system, PKC alpha influences synaptic plasticity and long term potentiation.

Phospho-PKC alpha Thr497 antibody offered by NSJ Bioreagents provides strong specificity for activated PKC alpha, supporting studies across oncology, cardiology, and neuroscience. It is a critical reagent for examining phosphorylation dependent signaling in health and disease.

UniProt

P17252

途径

WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling