C10orf2 抗体 (AA 39-635)
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Quick Overview for C10orf2 抗体 (AA 39-635) (ABIN7874604)
抗原
See all C10orf2 (C10ORF2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 39-635
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原理
- TWNK Antibody / Twinkle
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human Twinkle/TWNK recombinant protein (Position: R39-K635) was used as the immunogen for the TWNK antibody.
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亚型
- IgG
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anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (Middle Region) antibody
C10ORF2 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 犬, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (AA 559-684) antibodyC10ORF2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (C-Term) antibodyC10ORF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (AA 385-684) antibodyC10ORF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (AA 656-684), (C-Term) antibodyC10ORF2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32016 unconjugated
anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) antibodyC10ORF2 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (AA 591-684) antibodyC10ORF2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C5 unconjugated
anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (C-Term) antibodyC10ORF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (AA 540-589) antibodyC10ORF2 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 犬, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Chromosome 10 Open Reading Frame 2 (C10ORF2) (AA 612-661) antibodyC10ORF2 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the TWNK antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the TWNK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- C10orf2 (C10ORF2) (Chromosome 10 Open Reading Frame 2 (C10ORF2))
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别名
- TWNK
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背景
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TWNK antibody detects Twinkle, a mitochondrial DNA helicase essential for mtDNA replication and maintenance. Encoded by the TWNK gene on chromosome 10q24.31, this nuclear-encoded protein is imported into mitochondria, where it unwinds double-stranded DNA to facilitate replication of the mitochondrial genome. Twinkle is a member of the replicative helicase family related to bacteriophage T7 gene 4 helicase-primase, and it plays a vital role in mtDNA stability and copy number control.
Structurally, Twinkle forms a hexameric ring that encircles DNA and uses ATP hydrolysis to unwind duplex strands at replication forks. It cooperates with mitochondrial DNA polymerase gamma (POLG) and mitochondrial single-stranded DNA-binding protein (mtSSB) to sustain replication fidelity and genome integrity. Twinkle localizes to mitochondrial nucleoids, the structures where replication and transcription occur.
The TWNK antibody is widely used in mitochondrial biology, genetics, and metabolic research to study mtDNA replication, maintenance, and disorders of mitochondrial dysfunction. Western blot analysis detects a 77 kilodalton band corresponding to Twinkle, while immunofluorescence reveals mitochondrial localization with a punctate pattern. This antibody supports investigations into mechanisms of mtDNA propagation and repair.
Mutations in TWNK cause autosomal dominant progressive external ophthalmoplegia (PEO) and mitochondrial DNA depletion syndromes, leading to neuromuscular and metabolic disorders. Dysfunctional Twinkle results in replication stalling, deletions, and depletion of mitochondrial DNA. The TWNK antibody provides a reliable tool for studying mitochondrial replication machinery, genome stability, and related diseases. NSJ Bioreagents validates this antibody for western blotting, immunohistochemistry, and immunofluorescence, ensuring accurate detection for mitochondrial research. -
UniProt
- Q96RR1
抗原
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