RAD51 Homolog B 抗体 (AA 22-384)
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北京 101111
Quick Overview for RAD51 Homolog B 抗体 (AA 22-384) (ABIN7872323)
抗原
See all RAD51 Homolog B (Rad51B) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 22-384
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原理
- RAD51B Antibody / RAD51L1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human RAD51L1/RAD51B recombinant protein (Position: H22-F384) was used as the immunogen for the RAD51B antibody.
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亚型
- IgG
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anti-RAD51 Homolog B (Rad51B) antibody
Rad51B 适用: 人, 小鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAD51 Homolog B (Rad51B) (Middle Region) antibodyRad51B 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-RAD51 Homolog B (Rad51B) (AA 1-384) antibodyRad51B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-RAD51 Homolog B (Rad51B) (AA 143-192) antibodyRad51B 适用: 人, 猴, 犬, 豚鼠, 兔 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RAD51 Homolog B (Rad51B) (AA 251-300) antibodyRad51B 适用: 人, 小鼠, 猴, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-RAD51 Homolog B (Rad51B) (AA 1-384) antibodyRad51B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RAD51 Homolog B (Rad51B) (Center) antibodyRad51B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RAD51 Homolog B (Rad51B) (AA 201-250) antibodyRad51B 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the RAD51B antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the RAD51B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- RAD51 Homolog B (Rad51B)
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别名
- RAD51B
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背景
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RAD51B antibody detects DNA repair protein RAD51 homolog 2, encoded by the RAD51B gene (also known as RAD51L1) on chromosome 14q24.1. RAD51B antibody is widely used in DNA damage response, homologous recombination, and cancer biology research. RAD51B is part of the RAD51 paralog family, which functions in homologous recombination repair of DNA double-strand breaks. It acts as a mediator, promoting the assembly and stability of RAD51 nucleoprotein filaments on single-stranded DNA.
Structurally, RAD51B is a ~42 kDa protein with ATP-binding motifs and DNA-binding domains. It forms a heterodimer with RAD51C and participates in the BCDX2 complex with RAD51C, RAD51D, and XRCC2, as well as in the CX3 complex with RAD51C and XRCC3. These complexes support RAD51 loading at DNA damage sites and stabilize recombination intermediates.
Functionally, RAD51B contributes to DNA repair fidelity by ensuring accurate homologous recombination. Loss of RAD51B impairs recombination, leading to chromosomal instability, hypersensitivity to DNA-damaging agents, and defective cell cycle checkpoints. Researchers use RAD51B antibody to study DNA repair, recombination, and genome stability.
Clinically, RAD51B variants are associated with breast cancer susceptibility and other malignancies. Disruption of RAD51B function contributes to genomic instability, a hallmark of cancer. Because homologous recombination is a therapeutic target in oncology, RAD51B is of interest in studies of PARP inhibitor sensitivity and synthetic lethality. NSJ Bioreagents provides RAD51B antibody for DNA repair and cancer research.
Experimentally, RAD51B antibody is applied in western blotting to detect the ~42 kDa protein, in immunofluorescence to study DNA repair foci, and in co-immunoprecipitation to analyze protein complexes. Chromatin immunoprecipitation with RAD51B antibody supports studies of recombination site localization. -
UniProt
- O15315
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途径
- DNA Damage Repair
抗原
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