Melanoma gp100 抗体 (AA 182-5565)
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Quick Overview for Melanoma gp100 抗体 (AA 182-5565) (ABIN7871468)
抗原
See all Melanoma gp100 (PMEL) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 182-5565
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原理
- PMEL Antibody / Premelanosome protein / SILV
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SILV/PMEL recombinant protein (Position: H182-Q5565) was used as the immunogen for the PMEL antibody.
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亚型
- IgG
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anti-Premelanosome Protein (PMEL) antibody
PMEL 适用: 人, 大鼠, 猴, 犬 IHC, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 10E5 unconjugated
anti-Premelanosome Protein (PMEL) (AA 376-502) antibodyPMEL 适用: 人 IHC, WB, ELISA 宿主: 小鼠 Monoclonal PMEL-2037 unconjugated
anti-Premelanosome Protein (PMEL) (C-Term) antibodyVerified PMEL 适用: 人, 小鼠 WB, IF, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC, WB, FACS, IF 宿主: 小鼠 Monoclonal HMB45 unconjugated
anti-Premelanosome Protein (PMEL) (N-Term) antibodyPMEL 适用: 人, 马, 大鼠, 小鼠, 犬, Cow, 山羊, 豚鼠, 兔 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人, 大鼠, 小鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (C-Term) antibodyPMEL 适用: 人, 小鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC, WB 宿主: 兔 Monoclonal PMEL-1825R unconjugated Recombinant Antibody
anti-Premelanosome Protein (PMEL) (AA 25-467) antibodyPMEL 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC 宿主: 兔 Monoclonal 617R unconjugated Recombinant Antibody
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应用备注
- Optimal dilution of the PMEL antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PMEL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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别名
- PMEL
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背景
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PMEL antibody detects Premelanosome protein, a structural component of melanosomes essential for melanin synthesis and pigment formation. The UniProt recommended name is Premelanosome protein (PMEL). Also known as SILV, gp100, or ME20, this glycoprotein forms amyloid fibrils that serve as scaffolds for melanin polymerization within developing melanosomes.
Functionally, PMEL antibody identifies a 661-amino-acid type I transmembrane glycoprotein synthesized in melanocytes. PMEL is cleaved by proprotein convertases into M alpha and M beta fragments, which assemble into amyloid fibrils in stage II melanosomes. These fibrils provide a matrix for eumelanin deposition, enabling proper pigment granule maturation.
The PMEL gene is located on chromosome 12p13.31 and is expressed exclusively in pigment-producing cells, including melanocytes and retinal pigment epithelium. PMEL expression is regulated by the transcription factor MITF and is upregulated during melanocyte differentiation. It plays a central role in pigment biogenesis and melanosome architecture.
Pathologically, PMEL is a target antigen in melanoma immunotherapy and an established marker for melanocytic tumors. Mutations in PMEL can cause pigment dilution phenotypes in animals due to altered fibril formation. Research using PMEL antibody supports studies in pigmentation biology, melanoma diagnostics, and organelle structure.
PMEL antibody is validated for immunohistochemistry, western blotting, and immunofluorescence to detect melanosomal structural proteins. NSJ Bioreagents provides PMEL antibody reagents optimized for pigment biology, cancer research, and cell differentiation studies.
Structurally, Premelanosome protein contains an N-terminal signal peptide, a polycystic kidney disease-like (PKD) domain, and a transmembrane domain. Its cleaved fragments aggregate to form functional amyloid fibrils. This antibody supports detailed examination of PMEL's role in melanosome assembly and pigmentation control. -
UniProt
- P40967
抗原
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