MAML1 抗体 (AA 1-998)
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Quick Overview for MAML1 抗体 (AA 1-998) (ABIN7869672)
抗原
See all MAML1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-998
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原理
- MAML1 Antibody / Mastermind-like protein 1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human MAML1 recombinant protein (Position: M1-K998) was used as the immunogen for the MAML1 antibody.
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亚型
- IgG
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anti-Mastermind-Like 1 (MAML1) (N-Term) antibody
MAML1 适用: 人, 小鼠 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 1 (MAML1) (N-Term) antibodyVerified MAML1 适用: 人 ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Mastermind-Like 1 (MAML1) (AA 1-45) antibodyMAML1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 1 (MAML1) (AA 198-225) antibodyMAML1 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB18757 unconjugated
anti-Mastermind-Like 1 (MAML1) (AA 655-754) antibodyMAML1 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Mastermind-Like 1 (MAML1) (AA 75-125) antibodyMAML1 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 1 (MAML1) (AA 75-125) antibodyMAML1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 1 (MAML1) (AA 205-234), (Middle Region) antibodyMAML1 适用: 人 WB, FACS, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the MAML1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the MAML1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- MAML1 (Mastermind-Like 1 (MAML1))
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别名
- MAML1
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背景
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MAML1 antibody detects Mastermind-like protein 1, a transcriptional coactivator that plays a key role in the Notch signaling pathway, which regulates cell fate determination, differentiation, and proliferation. Encoded by the MAML1 gene on chromosome 5q35.3, this nuclear protein acts as a core component of the Notch transcriptional activation complex. Upon Notch receptor activation and nuclear translocation of the Notch intracellular domain (NICD), MAML1 binds NICD and the transcription factor CSL (CBF1/RBPJ), forming a ternary complex that recruits additional coactivators to initiate target gene transcription.
MAML1 contains an N-terminal basic domain responsible for binding NICD and CSL, a central acidic transcriptional activation domain, and a C-terminal domain that mediates interactions with additional transcription factors and coactivators such as p300/CBP. Through these interactions, MAML1 promotes transcription of Notch target genes including HES1, HEY1, and MYC, which are involved in cell differentiation and proliferation. Because Notch signaling is crucial for tissue development and homeostasis, MAML1 serves as a master regulator of developmental pathways across multiple cell types, including neural, hematopoietic, and epithelial lineages.
The MAML1 antibody is widely used in molecular biology, developmental biology, and cancer research to assess Notch pathway activation and transcriptional regulation. Western blot analysis typically identifies a 115 kilodalton band corresponding to MAML1, while immunofluorescence reveals nuclear localization that overlaps with Notch pathway components. This antibody enables the study of transcriptional coactivation events and gene regulation in Notch-mediated signaling contexts.
Beyond Notch signaling, MAML1 also functions as a coactivator for other transcriptional regulators such as MEF2C, p53, and beta-catenin, broadening its impact on gene networks related to cell growth and apoptosis. Dysregulation of MAML1 has been linked to oncogenic transformation, particularly in leukemia, glioma, and breast cancer, where aberrant Notch signaling drives uncontrolled proliferation. The MAML1 antibody provides a reliable tool for investigating transcriptional complexes and signaling crosstalk mechanisms. NSJ Bioreagents offers this antibody validated for its applications, ensuring reproducibility and specificity for transcriptional and signaling studies. -
UniProt
- Q92585
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途径
- Notch Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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