NOXO1 抗体 (AA 1-294)
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Quick Overview for NOXO1 抗体 (AA 1-294) (ABIN7869065)
抗原
See all NOXO1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-294
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原理
- NOXO1 Antibody / NADPH oxidase organizer 1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NOXO1 recombinant protein (Position: M1-E294) was used as the immunogen for the NOXO1 antibody.
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亚型
- IgG
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anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 304-376) antibody
NOXO1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (C-Term) antibodyVerified NOXO1 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 1-100) antibodyNOXO1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (C-Term) antibodyNOXO1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 121-370) antibodyNOXO1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (C-Term) antibodyNOXO1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 192-219) antibodyNOXO1 适用: 人 WB 宿主: 兔 Polyclonal RB37554 unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 238-252) antibodyNOXO1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NOXO1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NOXO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))
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别名
- NOXO1
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背景
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NOXO1 antibody detects NADPH oxidase organizer 1, a cytosolic adaptor protein that regulates reactive oxygen species (ROS) production by the NADPH oxidase complex. The UniProt recommended name is NADPH oxidase organizer 1 (NOXO1). This protein is a functional homolog of p47phox and is required for assembling and activating NADPH oxidase in nonphagocytic cells.
Functionally, NOXO1 antibody identifies a 376-amino-acid protein containing PX (phox homology) and SH3 (Src homology 3) domains that mediate membrane localization and protein-protein interactions. NOXO1 binds to membrane subunits such as NOX1, NOXA1, and p22phox, organizing them into an active complex that transfers electrons from NADPH to oxygen, generating superoxide. This process supports cellular signaling, host defense, and redox homeostasis.
The NOXO1 gene is located on chromosome 16q22.1 and encodes a regulatory component expressed in epithelial cells of the colon, prostate, and vascular tissues. Unlike its phagocytic counterpart p47phox, NOXO1 lacks an autoinhibitory region, resulting in constitutive activity under resting conditions. Its expression enables sustained ROS generation in nonimmune cells for physiological signaling functions such as cell proliferation and differentiation.
In redox signaling, NOXO1-derived ROS modulate kinase activation, ion transport, and gene expression. In the intestine, NOXO1 contributes to mucosal defense and microbial balance by supporting NOX1-mediated oxidative activity. In vascular and neural tissues, it influences smooth muscle tone and neuronal excitability. Dysregulation of NOXO1 expression or complex assembly has been implicated in oxidative stress-related disorders, including inflammatory bowel disease and hypertension.
NOXO1 antibody is widely used in redox biology, signaling, and oxidative stress research. It is suitable for western blotting, immunofluorescence, and co-immunoprecipitation to analyze NOXO1 expression and complex formation. This antibody aids in studying NADPH oxidase regulation, reactive oxygen generation, and oxidative signaling pathways. It is also useful in exploring NOX1 and NOX3 complex activity in epithelial and neuronal systems.
Structurally, NOXO1 features an N-terminal PX domain that binds phosphoinositides and C-terminal SH3 motifs that engage proline-rich regions of target proteins. Post-translational regulation includes phosphorylation and redox-dependent modifications that modulate its interaction with NOXA1 and NOX1. NSJ Bioreagents provides NOXO1 antibody reagents validated for use in redox signaling, ROS metabolism, and membrane complex assembly studies. -
UniProt
- Q8NFA2
抗原
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