VEGF 抗体
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北京 101111
Quick Overview for VEGF 抗体 (ABIN7865754)
抗原
See all VEGF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-Vascular Endothelial Growth Factor (VEGF) mouse monoclonal, VEGF-21, purified
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纯化方法
- affinity chromatography
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免疫原
- human VEGF189 recombinant protein
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亚型
- IgG1 kappa
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应用备注
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ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml
IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- VEGF (Vascular Endothelial Growth Factor (VEGF))
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背景
- VEGF-21 reacts with Vascular Endothelial Growth Factor, also known as Vascular Permeability Factor (VEGF/ VPF) and is the key mediator of angiogenesis. The MWs are 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. In addition to endothelial cells, VEGF and VEGF receptors are expressed on numerous non-endothelial cells including tumor cells. Positive control: Astrocytomas, breast or ovarian carcinomas.
抗原
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