VEGF 抗体
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北京 101111
Quick Overview for VEGF 抗体 (ABIN2443873)
抗原
See all VEGF 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- VEGF Chicken Polyclonal Antibody
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特异性
- The antibody recognizes the partially reduced homodimmers and multimers that VEGF spontaneously forms. In reduced samples, the antibody recognizes the reduced monomer.
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纯化方法
- Purified
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免疫原
- Recombinant human VEGF
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anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-120) antibody
VEGF 适用: 人, 小鼠, 大鼠, Pig, 山羊 WB, ELISA, IHC (p), FACS, IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 大鼠, 犬, Pig, Cow, 小鸡 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) (C-Term) antibodyVEGF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB11705 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 大鼠 WB, IHC, ELISA, FACS, IP, BR 宿主: 小鼠 Monoclonal A-4-6-1 unconjugated Recombinant Antibody
anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-233) antibodyVEGF 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal 4E3 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 灵长类 WB, IHC, ELISA 宿主: 小鼠 Monoclonal VG76e unconjugated Recombinant Antibody
anti-Vascular Endothelial Growth Factor (VEGF) (N-Term) antibodyVEGF 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-233) antibodyVEGF 适用: 人, 犬 WB, IHC 宿主: 小鼠 Monoclonal 4G3 unconjugated
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应用备注
- WB 1:1000,IF 1:500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 30 μL: WB 1:1000 - 1:8000, IF 1:500 - 1:2000 (in cells fixed with paraformaldehyde). Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized purified total IgY in PBS pH 7.4
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储存液
- Sodium azide, Streptomycin
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- VEGF (Vascular Endothelial Growth Factor (VEGF))
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别名
- VEGF
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背景
- Vascular endothelial growth factor (VEGF) is a dimeric glycoprotein that stimulates vasculogenesis and angiogenesis. VEGF belongs to a family of closely related growth factors that share a conserved pattern of eight cysteine residues and bind to common VEGF receptors. The VEGF family members bind in an overlapping manner to three receptor tyrosine kinases, VEGFR1, VEGFR2 and VEGFR3. Specifically, VEGF mediates angiogenic response by the activation of VEGFR2. VEGF is produced at high levels in endothelial cells but its expression can be found in other cell types, including fibroblasts, inflammatory cells, and many tumor cells, often in response to increasing tumor hypoxia via the HIF-1a pathway. Overexpression of VEGF can contribute to diseases such as cancer and vascular disease in the retina, also, VEGF is a cause of susceptibility to microvascular complications of diabetes type 1 such as diabetic retinopathy, diabetic nephropathy and diabetic neuropathy.
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UniProt
- P15692
抗原
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