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TGFA 抗体

This 小鼠 单克隆 antibody specifically detects TGFA in IHC, FACS 和 ELISA. It exhibits reactivity toward 人, 兔 和 斑马鱼.
产品编号 ABIN7865750
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for TGFA 抗体 (ABIN7865750)

抗原

See all TGFA 抗体
TGFA (Transforming Growth Factor, alpha (TGFA))

适用

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人, 兔, 斑马鱼

宿主

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  • 3
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小鼠

克隆类型

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单克隆

标记

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This TGFA antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

克隆位点

1E8-G6
  • 原理

    anti-TGF-alpha mouse monoclonal, 1E8-G6, purified

    纯化方法

    affinity chromatography

    免疫原

    synthetic TGF-alpha 34-43 decapeptide linked carrier

    亚型

    IgM kappa
  • 应用备注

    FACS_dilution: 0.5-1,0 μg/million cells in 0.1 ml

    IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

    WB_dilution: 1:50-1:100 (1-2 μg/mL)

    IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

    elisa_dilution: assay dependent

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS with 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    store at 2 - 8 °C
  • 抗原

    TGFA (Transforming Growth Factor, alpha (TGFA))

    别名

    TGF-alpha

    背景

    1E8-G6 reacts with TGF-alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. 1E8-G6 is completely blocked by the peptide used for immunization. TGF-alpha (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. In sections both cytoplasma and cell membranes are stained. Positive control: Jurkat or Ramos cells. testicular carcinoma, breast cancer.

    途径

    NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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