TGFA 抗体
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北京 101111
Quick Overview for TGFA 抗体 (ABIN7865750)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-TGF-alpha mouse monoclonal, 1E8-G6, purified
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纯化方法
- affinity chromatography
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免疫原
- synthetic TGF-alpha 34-43 decapeptide linked carrier
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亚型
- IgM kappa
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应用备注
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FACS_dilution: 0.5-1,0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
elisa_dilution: assay dependent
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGF-alpha
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背景
- 1E8-G6 reacts with TGF-alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. 1E8-G6 is completely blocked by the peptide used for immunization. TGF-alpha (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. In sections both cytoplasma and cell membranes are stained. Positive control: Jurkat or Ramos cells. testicular carcinoma, breast cancer.
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原
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