Melanoma gp100 抗体
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北京 101111
Quick Overview for Melanoma gp100 抗体 (ABIN7865716)
抗原
See all Melanoma gp100 (PMEL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-Pmel17/gp100 mouse monoclonal, EBS-O-147, purified
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纯化方法
- affinity chromatography
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免疫原
- fresh metatstatic melanotic melanoma cell membranes
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亚型
- IgG2b kappa
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应用备注
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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别名
- Pmel17/gp100
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背景
- EBS-O-147 recognizes an intracellular antigen (innerside of the membrane of melanosomes in cultured cells) of 90-100 kDa in cells of the melanocyte lineage (gp100 as well as related pMel17). It is positive with skin melanomas, choroidal melanomas, uveal melanomas, melanoma metastases, skin melanocytes, common and dysplastic nevi, negative with all other tumors and normal tissues also with intradermal nevi and normal adult melanocytes. Positive control: SK-MEL-28 or melanoma.
抗原
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