HSPD1 抗体
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北京 101111
Quick Overview for HSPD1 抗体 (ABIN7865645)
抗原
See all HSPD1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-HSP60 mouse monoclonal, HSP60-1, purified
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纯化方法
- affinity chromatography
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免疫原
- recombinant human HSPD1
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亚型
- IgG1 kappa
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应用备注
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
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别名
- HSP60
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背景
- HSP60-1 reacts with Heat Shock Protein 60 or HSPD1, found in mitochondria. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (HSP’s), more appropriately called as Stress Response Proteins (SRP’s). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian. Positive control: HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Carcinomas.
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途径
- Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
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