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CD51 抗体 (FITC)

The FITC-conjugated 小鼠 单克隆 anti-CD51 antibody (Clone 13C2) (ABIN7836446) specifically detects CD51 in FACS 和 IF. The antibody is reactive with 人 samples.
产品编号 ABIN7836446
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD51 抗体 (FITC) (ABIN7836446)

抗原

See all CD51 (ITGAV) 抗体
CD51 (ITGAV) (Integrin alpha V (ITGAV))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD51 antibody is conjugated to FITC

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF)

克隆位点

13C2
  • 原理

    Mouse monoclonal antibody FITC labled to CD51

    特异性

    Recognizes human CD51

    纯化方法

    The antibody was purified by affinity chromatography.

    免疫原

    Cell suspension containing osteoclasts from osteoclastomas
  • 应用备注

    Flow: 10 μL / assay

    实验流程

    1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Shipped and store at 4°C for one year. Do not freeze.
  • 抗原

    CD51 (ITGAV) (Integrin alpha V (ITGAV))

    别名

    ITGAV

    背景

    MSK8, VNRA, Integrin alpha-V, Vitronectin receptor subunit alpha, CD51

    基因ID

    3685

    UniProt

    P06756

    途径

    Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex
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