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RFP 抗体

The 兔 多克隆 anti-RFP antibody (ABIN7833506) specifically detects RFP in WB 和 IHC. The antibody is reactive with Discosoma 和 Aequorea victoria samples.
产品编号 ABIN7833506
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for RFP 抗体 (ABIN7833506)

抗原

See all RFP 抗体
RFP (Red Fluorescent Protein (RFP))

适用

  • 60
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Discosoma, Aequorea victoria

宿主

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  • 1

克隆类型

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多克隆

标记

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This RFP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 原理

    Anti-RFP | Red flourescent protein (mRFP, mCherry, tdTomato, mScarlet)

    交叉反应 (详细)

    RFP, mCherry-YFP
    Not reactive in: Sequence identity with GFP is very low and it is therefore expected that this antibody does not cross-reacti with GFP protein.

    预测反应

    mRFP,mCherry,tdTomato,mScarlet

    纯化方法

    Antigen affinity purified serum

    免疫原

    Recombinant protein produced in E.coli corresponding to full lenght mRFP. The sequence used for immunization is also found in other red flourescent proteins like: mCherry/tdTomato/mScarlet and others.
  • 应用备注

    1: 200 (IHC), 1: 2500 (WB)

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution, add 50 μL, of sterile or deionized water.

    缓冲液

    Antigen affinity purified serum, in PBS pH 7.4

    储存条件

    -20 °C

    储存方法

    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • 抗原

    RFP (Red Fluorescent Protein (RFP))

    别名

    RFP

    背景

    Red fluorescent protein (RFP) once excited is fluorescing red-orange light. The mass of RFP is approximately 25.9 kDa and its excitation maximum is 558 nm and emission maximum is 583 nm. Improved variants of RFP include so called mFruits variants: mCherry, mOrange, mRaspberry.

    分子量

    Depends upon fusion partner
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