RFP 抗体
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北京 101111
Quick Overview for RFP 抗体 (ABIN7833506)
抗原
See all RFP 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-RFP | Red flourescent protein (mRFP, mCherry, tdTomato, mScarlet)
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交叉反应 (详细)
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RFP, mCherry-YFP
Not reactive in: Sequence identity with GFP is very low and it is therefore expected that this antibody does not cross-reacti with GFP protein. -
预测反应
- mRFP,mCherry,tdTomato,mScarlet
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纯化方法
- Antigen affinity purified serum
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免疫原
- Recombinant protein produced in E.coli corresponding to full lenght mRFP. The sequence used for immunization is also found in other red flourescent proteins like: mCherry/tdTomato/mScarlet and others.
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应用备注
- 1: 200 (IHC), 1: 2500 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution, add 50 μL, of sterile or deionized water.
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缓冲液
- Antigen affinity purified serum, in PBS pH 7.4
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- RFP (Red Fluorescent Protein (RFP))
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别名
- RFP
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背景
- Red fluorescent protein (RFP) once excited is fluorescing red-orange light. The mass of RFP is approximately 25.9 kDa and its excitation maximum is 558 nm and emission maximum is 583 nm. Improved variants of RFP include so called mFruits variants: mCherry, mOrange, mRaspberry.
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分子量
- Depends upon fusion partner
抗原
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