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LC3B 抗体

The 兔 多克隆 anti-LC3B antibody is suitable to detect LC3B in samples from Zea mays, Tomato, Poplar (Populus), Nicotiana benthamiana, Haematococcus lacustris, Gossypim hirsutum, Chlorococcum sp. 'dorsiventrale', Chlamydomonas reinhardtii, Arabidopsis thaliana 和 Aponogeton madagascariensis. It has been validated for WB 和 IL.
产品编号 ABIN7833424
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for LC3B 抗体 (ABIN7833424)

抗原

See all LC3B (MAP1LC3B) 抗体
LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

适用

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Zea mays, Tomato, Poplar (Populus), Nicotiana benthamiana, Haematococcus lacustris, Gossypim hirsutum, Chlorococcum sp. 'dorsiventrale', Chlamydomonas reinhardtii, Arabidopsis thaliana, Aponogeton madagascariensis

宿主

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克隆类型

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多克隆

标记

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This LC3B antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunolocalization (IL)
  • 原理

    Anti-ATG8 | Autophagy-related protein

    交叉反应 (详细)

    Arabidopsis thaliana, Aponogeton madagascariensis, Chlamydononas reinhardtii, Chlorococcum dorsiventrale, Gossypim hirsutum, Haematococcus lacustris, Nicotiana benthamiana, Populus trichocarpa, Solanum lycopersicum, Zea mays
    Not reactive in: Cuscuta chinensis, Symbiodinium sp.

    预测反应

    Ananas comosus,Brassica napus,Hordeum vulgare,Micromonas sp.,Nelumbo nucifera,Oryza sativa,Panicum hallii,Parachlorella kessleri,Phoenix dactylifera,Pyrus x bretschneideri,Physcomitrium patens,Pinus sitchensis,Solanum tuberosum,Triticum aestivum,Volvox carteri

    纯化方法

    Serum

    免疫原

    Fragment of recombinant ATG8 from Chlamydomonas reinhardtii
  • 应用备注

    1 : 1000 (IL), 1 : 1000-1 : 2000 (WB)

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 50 μL of sterile water

    缓冲液

    Serum

    注意事项

    Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

    储存条件

    -20 °C

    储存方法

    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • 抗原

    LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

    别名

    ATG8

    背景

    Background: ATG8 (Autophagy-related protein 8) is involved in degradation and recycling of intracellular components in a process of autophagy. ATG8 is a molecular autophagy marker in Chlamydomonas reinhardtii (Pérez-Pérez et al. 2010, Plant Physiol. 152: 1874-88).

    Additional information: This product can be sold containing ProClin if requested.This antibody is recognizing 1 ng of recombinant CrATG8. Antigen used to elicit this antibody is conserved from 70-80 % in following ATG protein from Arabidopsis thaliana: ATG8a UniProt: Q8LEM4 ATG8B UniProt: Q9XEB5 ATG8c UniProt: Q8S927 ATG8d UniProt: Q9SL04, ATG8e UniProt: Q8S926 ATG8f UniProt: Q8VYK7 and conserved below 70 % in: ATG8g UniProt: Q9LZZ9 ATG8h Uniprot: Q8S92This antibody does not recognize all isoforms into the same degree.

    分子量

    15.2 | 15 kDa

    基因ID

    5724800

    UniProt

    A8JB85

    途径

    Autophagy
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