Recombinant MCM5 抗体
Quick Overview for Recombinant MCM5 抗体 (ABIN7826129)
抗原
See all MCM5 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- MCM5 antibody (505R)
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免疫原
- Recombinant human MCM5 fragment
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: A strong nuclear MCM5 immunostaining should be seen in virtually all crypt base cells.
Product Description/Background: MCM5 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: MCM5 immunostaining should be less intense or absent in surface epithelial cells and absent in most stroma cells.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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有效期
- 24 months
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- MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))
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别名
- MCM5
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背景
- Gene Alias: Minichromosome maintenance complex component 5, CDC46, MGORS8, P1-CDC46
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UniProt
- P339922
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途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
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