Recombinant CDKN1B 抗体
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北京 101111
Quick Overview for Recombinant CDKN1B 抗体 (ABIN7826114)
抗原
See all CDKN1B 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- P27 / Kip1 antibody (HMV3970)
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免疫原
- Recombinant human CDKN1B fragment
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Tonsil: A strong nuclear p27 staining should be seen in a small subset of germinal centre cells and in a large fraction of interfollicular lymphocytic cells.
Product Description/Background: P27 / Kip1 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Tonsil: p27 staining should be completely absent in most germinal centre cells.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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有效期
- 24 months
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- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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别名
- P27 / Kip1
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背景
- Gene Alias: cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1
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UniProt
- P46527
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
抗原
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