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SPARC 抗体 (AA 101-200)

Name: SPARC 抗体 (AA 101-200)
SKU: ABIN738141
Availability: OutOfStock

This anti-SPARC antibody is a 兔多克隆 antibody detecting SPARC in WB, ELISA, IHC (p), IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 3+ publications.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-SPARC Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (SPARC 抗体 (AA 101-200))

IHC

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-SPARC Polyclonal Antibody, Unconjugated (ABIN738141) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (SPARC 抗体 (AA 101-200))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with SPARC Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (SPARC 抗体 (AA 101-200))

IHC (p)

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-SPARC Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (SPARC 抗体 (AA 101-200))

13 images

(3 references)

产品编号 ABIN738141

发货至: 中国

Quick Overview for SPARC 抗体 (AA 101-200) (ABIN738141)

抗原

See all SPARC 抗体

SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This SPARC antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

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AA 101-200

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Pig,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human SPARC

亚型

IgG
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibody
SPARC 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B2 unconjugated

anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251) antibody
SPARC 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody
SPARC 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303) antibody
SPARC 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251), (C-Term) antibody
SPARC 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19869 unconjugated

anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibody
SPARC 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) antibody
SPARC 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303), (C-Term) antibody
SPARC 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibody
SPARC 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-301) antibody
SPARC 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zong, Zeng, Zou, Li, Fang, Lu, Xiao, Zhang: "Effects of Polygonatum sibiricum polysaccharide on the osteogenic differentiation of bone mesenchymal stem cells in mice." in: International journal of clinical and experimental pathology, Vol. 8, Issue 6, pp. 6169-80, (2015) (PubMed).

Kurtul, Eroglu, Unal, Tasdemir, Orhan, Zararsiz, Baran, Kaplan, Kontas: "Prognostic Value of SPARC Expression in Unresectable NSCLC Treated with Concurrent Chemoradiotherapy." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 15, Issue 20, pp. 8911-6, (2014) (PubMed).

Chen, Wang, Xi, Xue, He, Zhang, Zhao: "SPARC is a key regulator of proliferation, apoptosis and invasion in human ovarian cancer." in: PLoS ONE, Vol. 7, Issue 8, pp. e42413, (2012) (PubMed).
抗原

SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

别名

SPARC

背景

Synonyms: ON, SPARC, Basement-membrane protein 4, BM-4, Osteonectin, Secreted protein acidic and rich in cysteine
Background: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites, an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

基因ID

6678

UniProt

P09486

途径

Autophagy