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IL12 抗体 (AA 51-150)

Name: IL12 抗体 (AA 51-150)
SKU: ABIN734453
Availability: OutOfStock

This anti-IL12 antibody is a 兔多克隆 antibody detecting IL12 in ELISA, WB, IF (p), IF (cc), IHC (fro) 和 IHC (p). Suitable for 人. This Primary Antibody has been cited in 1 publication.

ELISA

U251 lysates probed with IL12 Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (IL12 抗体 (AA 51-150))

paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-IL12 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (IL12 抗体 (AA 51-150))

IHC (p)

paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-IL12 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (IL12 抗体 (AA 51-150))

IHC (p)

IF (p)

5 images

(1 reference)

产品编号 ABIN734453

发货至: 中国

Quick Overview for IL12 抗体 (AA 51-150) (ABIN734453)

抗原

See all IL12 抗体

IL12 (Interleukin 12 (IL12))

适用

人

宿主

兔

克隆类型

多克隆

标记

This IL12 antibody is un-conjugated

应用范围

ELISA, Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

抗原表位
15

14

6

3

2

1

1

1

1

1

1

1
AA 51-150

交叉反应

人

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human IL-12

亚型

IgG
anti-Interleukin 12 (IL12) (AA 168-195) antibody
IL12 适用: 人 ELISA, WB, FACS, IHC 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 12 (IL12) (AA 51-150) antibody (AbBy Fluor® 555)
IL12 适用: 人 WB, IF (p), IF (cc) 宿主: 兔 Polyclonal AbBy Fluor® 555

anti-Interleukin 12 (IL12) antibody
IL12 适用: 人 ELISA, WB, Func 宿主: 山羊 Polyclonal unconjugated

anti-Interleukin 12 (IL12) antibody
IL12 适用: 小鼠 ELISA, WB, Neut 宿主: 山羊 Polyclonal unconjugated

anti-Interleukin 12 (IL12) antibody
IL12 适用: 人 ELISA, WB, Neut 宿主: 山羊 Polyclonal unconjugated

anti-Interleukin 12 (IL12) antibody
IL12 适用: 小鼠 ELISA, ELISpot 宿主: 大鼠 Monoclonal C17-8 unconjugated

anti-Interleukin 12 (IL12) antibody (Biotin)
IL12 适用: 小鼠 ELISA, ELISpot 宿主: 大鼠 Monoclonal IL12-IV Biotin

anti-Interleukin 12 (IL12) antibody
IL12 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal B-P40 unconjugated

anti-Interleukin 12 (IL12) antibody
IL12 适用: 人 ELISA, FACS, ELISpot 宿主: 小鼠 Monoclonal B-P24 unconjugated

anti-Interleukin 12 (IL12) antibody (Biotin)
IL12 适用: 人, Non-Human Primate ELISA, ELISpot 宿主: 小鼠 Monoclonal MT704 Biotin

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Li, Dan, Hu, Ouzhu, Ciren, Wang, Wang, Yang, Ze: "CD14 overexpression upregulates TNF-?-mediated inflammatory responses and suppresses the malignancy of gastric carcinoma cells." in: Molecular and cellular biochemistry, Vol. 376, Issue 1-2, pp. 137-43, (2013) (PubMed).
抗原

IL12 (Interleukin 12 (IL12))

别名

IL-12

背景

Synonyms: P35, CLMF, NFSK, NKSF1, IL-12A, Interleukin-12 subunit alpha, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, NK cell stimulatory factor chain 1, IL12A
Background: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.

基因ID

3592

UniProt

P29459

途径

JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome

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IL12 抗体 (AA 51-150)

Quick Overview for IL12 抗体 (AA 51-150) (ABIN734453)

抗原

适用

宿主

克隆类型

标记

应用范围

IL12抗体详情

抗原表位

交叉反应

纯化方法

免疫原

亚型

Alternatives

使用细节

应用备注

限制

贮存及处理

状态

浓度

缓冲液

储存液

注意事项

储存条件

储存方法

有效期

References for anti-IL12 antibody (ABIN734453)

IL12目标详情

抗原

别名

背景

基因ID

UniProt

途径