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TGFA 抗体 (CF®740)

This anti-TGFA antibody is a 小鼠 单克隆 antibody detecting TGFA in IHC 和 IHC (fp). Suitable for 人.
产品编号 ABIN7661059
发货至: 中国

Quick Overview for TGFA 抗体 (CF®740) (ABIN7661059)

抗原

See all TGFA 抗体
TGFA (Transforming Growth Factor, alpha (TGFA))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This TGFA antibody is conjugated to CF®740

应用范围

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Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

TGFA-1119
  • 原理

    TGFalpha(TGFA/1119), CF740 conjugate

    产品特性

    This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGF-beta (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human full-length TGF alpha protein

    亚型

    IgG1, kappa
  • 应用备注

    Immunohistology formalin-paraffin 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: Jurkat or Ramos cells. Testicular Carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    TGFA (Transforming Growth Factor, alpha (TGFA))

    别名

    TGF-Alpha

    背景

    Synonyms: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

    Gene Symbol: TGFA

    分子量

    6 kDa

    基因ID

    7039

    UniProt

    P01135

    途径

    NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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