TGFA 抗体 (CF®740)
Quick Overview for TGFA 抗体 (CF®740) (ABIN7661059)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- TGFalpha(TGFA/1119), CF740 conjugate
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产品特性
- This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGF-beta (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human full-length TGF alpha protein
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亚型
- IgG1, kappa
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应用备注
- Immunohistology formalin-paraffin 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Jurkat or Ramos cells. Testicular Carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGF-Alpha
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背景
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Synonyms: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1
Gene Symbol: TGFA
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分子量
- 6 kDa
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基因ID
- 7039
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UniProt
- P01135
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原
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