SOX4 抗体 (CF®405S)
Quick Overview for SOX4 抗体 (CF®405S) (ABIN7660312)
抗原
See all SOX4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- SOX4 (Master Regulator of Epithelial-Mesenchymal Transition) (SOX4/2540), CF405S conjugate
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产品特性
- SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors with a critical role in embryonic development and in cell-fate determination during organogenesis of the heart, pancreas, brain, and in B and T lymphocyte differentiation. SOX4 gene expression is upregulated in many cancer types, and increased SOX4 activity contributes to cellular transformation, cell survival, and metastasis. Gene expression profiling has uncovered SOX4 with upregulated activity during TGF-β-induced epithelial-mesenchymal transition (EMT) in normal and cancerous breast epithelial cells. SOX4 is indispensable for EMT and cell survival in vitro and for primary tumor growth and metastasis in vivo. SOX4 is identified as a master regulator of EMT by governing the expression of the epigenetic modifier Ezh2.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human SOX4 protein
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亚型
- IgG2b, kappa
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anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 54-103) antibody
SOX4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (N-Term) antibodySOX4 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 斑马鱼, 山羊, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 86-114) antibodySOX4 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) antibodySOX4 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal SOX4-2540 unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 86-114), (N-Term) antibodySOX4 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB18502 unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (N-Term) antibodySOX4 适用: 人, 小鼠, 大鼠, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 1-60) antibodySOX4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 45-136) antibodySOX4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 1-100) antibodySOX4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) antibodySOX4 适用: 人 ICC, DB 宿主: 小鼠 Monoclonal 154C4a unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Breast or Colon Carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))
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别名
- SOX4
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背景
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Synonyms: SOX, SRY (sex determining region Y) box 4, SRY box containing gene 4, SRY related HMG box gene 4, Transcription factor SOX-4
Gene Symbol: SOX4
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分子量
- 47 kDa
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基因ID
- 6659
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UniProt
- Q06945
抗原
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