L-Selectin 抗体 (Biotin)
Quick Overview for L-Selectin 抗体 (Biotin) (ABIN7659758)
抗原
See all L-Selectin (SELL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- CD62L (L-Selectin)(CD62L/1588), Biotin conjugate
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产品特性
- Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- MAb raised against supernatant from phorbol myristic acid activated human peripheral blood leukocytes.
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亚型
- IgG1, kappa
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应用备注
- For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry
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说明
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Positive Control: HPBL or Jurkat whole cell lysate.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- L-Selectin (SELL) (Selectin L (SELL))
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别名
- CD62L
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背景
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Synonyms: A.11, AI528707, CD62 antigen ligand, CD62L, gp90-MEL, IgA nephropathy, susceptibility to, included, L Selectin, L-selectin, LAM1, LECAM1, LEU8, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lnhr, LSEL, Ly-22, LYAM1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, Pln homing receptor, PLNHR, SELL, TQ1
Gene Symbol: SELL
Tissue Expression: Eosinophils|Lymphocytes|Monocytes/macrophages|Neutrophils
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分子量
- 65-75 kDa
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基因ID
- 6402
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UniProt
- P14151
抗原
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