L-Selectin 抗体 (CF®488A)
Quick Overview for L-Selectin 抗体 (CF®488A) (ABIN7659753)
抗原
See all L-Selectin (SELL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- CD62L (L-Selectin)(LAM1-116), CF488A conjugate
-
产品特性
- Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- MAb raised against supernatant from phorbol myristic acid activated human peripheral blood leukocytes.
-
亚型
- IgG1, kappa
-
-
-
-
应用备注
- ELISA: For coating, order antibody without BSA, Flow cytometry: 0.5-1 μg/million cells, Western blot 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
-
说明
-
Positive Control: HPBL or Jurkat whole cell lysate.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.1 mg/mL
-
缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
储存条件
- 4 °C
-
储存方法
-
Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
-
有效期
- 24 months
-
-
- L-Selectin (SELL) (Selectin L (SELL))
-
别名
- CD62L
-
背景
-
Synonyms: A.11, AI528707, CD62 antigen ligand, CD62L, gp90-MEL, IgA nephropathy, susceptibility to, included, L Selectin, L-selectin, LAM1, LECAM1, LEU8, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lnhr, LSEL, Ly-22, LYAM1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, Pln homing receptor, PLNHR, SELL, TQ1
Gene Symbol: SELL
Tissue Expression: Eosinophils|Lymphocytes|Monocytes/macrophages|Neutrophils
-
分子量
- 65-75 kDa
-
基因ID
- 6402
-
UniProt
- P14151
抗原
-