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L-Selectin 抗体 (CF®405S)

This anti-L-Selectin antibody is a 小鼠 单克隆 antibody detecting L-Selectin in FACS, WB, IHC, IP, ELISA, Func 和 IHC (fp). Suitable for 人.
产品编号 ABIN7659743
发货至: 中国

Quick Overview for L-Selectin 抗体 (CF®405S) (ABIN7659743)

抗原

See all L-Selectin (SELL) 抗体
L-Selectin (SELL) (Selectin L (SELL))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This L-Selectin antibody is conjugated to CF®405S

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Functional Studies (Func), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

LAM1-116
  • 原理

    CD62L (L-Selectin)(LAM1-116), CF405S conjugate

    产品特性

    Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    MAb raised against supernatant from phorbol myristic acid activated human peripheral blood leukocytes.

    亚型

    IgG1, kappa
  • 应用备注

    ELISA: For coating, order antibody without BSA, Flow cytometry: 0.5-1 μg/million cells, Western blot 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

    说明

    Positive Control: HPBL or Jurkat whole cell lysate.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % BSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    L-Selectin (SELL) (Selectin L (SELL))

    别名

    CD62L

    背景

    Synonyms: A.11, AI528707, CD62 antigen ligand, CD62L, gp90-MEL, IgA nephropathy, susceptibility to, included, L Selectin, L-selectin, LAM1, LECAM1, LEU8, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lnhr, LSEL, Ly-22, LYAM1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, Pln homing receptor, PLNHR, SELL, TQ1

    Gene Symbol: SELL

    Tissue Expression: Eosinophils|Lymphocytes|Monocytes/macrophages|Neutrophils

    分子量

    65-75 kDa

    基因ID

    6402

    UniProt

    P14151
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