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Prostate Specific Antigen 抗体 (CF®568)

The 小鼠 单克隆 anti-Prostate Specific Antigen antibody is suitable to detect Prostate Specific Antigen in samples from 人 和 Cat. It has been validated for IHC 和 IHC (fp).
产品编号 ABIN7659254
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
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Quick Overview for Prostate Specific Antigen 抗体 (CF®568) (ABIN7659254)

抗原

See all Prostate Specific Antigen (PSA) 抗体
Prostate Specific Antigen (PSA)

适用

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人, Cat

宿主

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小鼠

克隆类型

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单克隆

标记

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This Prostate Specific Antigen antibody is conjugated to CF®568

应用范围

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Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

1A7
  • 原理

    Prostate Specific Antigen (PSA)(1A7), CF568 conjugate

    产品特性

    This antibody recognizes a single protein of 33-34 kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This MAb makes an excellent pair with MAb A67-B/E3 for PSA tests.

    免疫原

    PSA (p30) from human sperm plasma

    亚型

    IgG1, kappa
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.5-1.0 μg/mL for 30 minutes at RT. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: PC-3 or LNCaP cell lysates (WB). Prostate tissue (IHC). LNCaP cells (IF).

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % BSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    Prostate Specific Antigen (PSA)

    别名

    Prostate Specific Antigen

    背景

    Synonyms: Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin

    Gene Symbol: KLK3

    Tissue Expression: Prostate

    分子量

    33-34 kDa

    基因ID

    354

    UniProt

    P07288

    途径

    Complement System
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