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Prostate Specific Antigen 抗体

PSA 适用: 人 ELISA, WB, FACS, DB 宿主: 小鼠 Monoclonal A5D5-1 unconjugated
产品编号 ABIN1543584
发货至: 中国
  • 抗原 See all Prostate Specific Antigen (PSA) 抗体
    Prostate Specific Antigen (PSA)
    适用
    • 189
    • 15
    • 10
    • 5
    • 4
    • 1
    宿主
    • 117
    • 65
    • 4
    • 4
    • 2
    小鼠
    克隆类型
    • 131
    • 61
    单克隆
    标记
    • 101
    • 17
    • 13
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Prostate Specific Antigen antibody is un-conjugated
    应用范围
    • 90
    • 76
    • 37
    • 37
    • 26
    • 25
    • 24
    • 14
    • 13
    • 9
    • 9
    • 9
    • 8
    • 5
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    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB)
    纯化方法
    Protein A affinity column
    免疫原
    Native PSA Antigen
    克隆位点
    A5D5-1
    亚型
    IgG1
  • 说明

    Other Applications have not been tested.

    限制
    仅限研究用
  • 生效 #029617 (Flow Cytometry)
    '独立验证'标志
    by
    Flow Cytometry & Cell Separation Facility, Purdue University
    No.
    #029617
    日期
    2014.02.22
    抗原
    Lot Number
    FAPS-A5D5-0003
    Method validated
    Flow Cytometry
    Positive Control
    LNCaP cells
    Negative Control
    - DU145 cells - Jurkat cells
    Notes
    Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antibody: Prostate Specific Antigen (PSA)
    • Catalog number: ABIN1543584
    • Lot number: FAPS-A5D5-0003
    Secondary Antibody
    • Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647
    Full Protocol
    • Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
    • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
    • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
    • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
    • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
    • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
    • 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
    • Cells were washed twice with PBS/BSA/Tween 20
    • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
    • Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
    • Cells were washed twice with PBS/BSA/Tween 20
    • Cells were resuspended in 0.3 mL PBS/BSA
    • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
    Experimental Notes
    None
  • 状态
    Liquid
    浓度
    1.8 mg/mL
    缓冲液
    PBS, pH 7.4, 0.09% sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
  • 抗原
    Prostate Specific Antigen (PSA)
    别名
    PSA (PSA 产品)
    别名
    APS antibody, KLK2A1 antibody, PSA antibody, hK3 antibody, KLK3 antibody, Klk1c10l2 antibody, RSGK-50 antibody, RSKG-50 antibody, rGK-3 antibody, Kal-3 antibody, Kal3 antibody, Klk3 antibody, AAP-S antibody, MP100 antibody, Psa antibody, R74825 antibody, goku antibody, kallikrein related peptidase 3 antibody, kallikrein 3 antibody, kallikrein 1-related peptidase C3 antibody, kallikrein B, plasma 1 antibody, aminopeptidase puromycin sensitive antibody, KLK3 antibody, Klk1c3 antibody, Klkb1 antibody, Npepps antibody
    途径
    Complement System
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