Prostate Specific Antigen 抗体 (CF®405S)
Quick Overview for Prostate Specific Antigen 抗体 (CF®405S) (ABIN7659236)
抗原
See all Prostate Specific Antigen (PSA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Prostate Specific Antigen (PSA)(1A7), CF405S conjugate
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产品特性
- This antibody recognizes a single protein of 33-34 kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This MAb makes an excellent pair with MAb A67-B/E3 for PSA tests.
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免疫原
- PSA (p30) from human sperm plasma
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亚型
- IgG1, kappa
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.5-1.0 μg/mL for 30 minutes at RT. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: PC-3 or LNCaP cell lysates (WB). Prostate tissue (IHC). LNCaP cells (IF).
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Prostate Specific Antigen (PSA)
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别名
- Prostate Specific Antigen
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背景
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Synonyms: Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin
Gene Symbol: KLK3
Tissue Expression: Prostate
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分子量
- 33-34 kDa
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基因ID
- 354
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UniProt
- P07288
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途径
- Complement System
抗原
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