POU2AF1 抗体 (AA 148-255) (CF®488A)
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北京 101111
Quick Overview for POU2AF1 抗体 (AA 148-255) (CF®488A) (ABIN7658795)
抗原
See all POU2AF1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 148-255
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原理
- BOB.1 (B-Cell Marker) (BOB1/2424), CF488A conjugate
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产品特性
- BOB-1 is a transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2, boosting the promoter activity of those complexes. It is essential for the response of B-cells to antigens, required for the formation of germinal centers, and regilates IL-6 expression. BOB-1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB-1. Conversely, only some cases of classical Hodgkin lymphoma show BOB-1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB-1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human BOB1 (POU2AF1) protein fragment (around aa 148-255)
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亚型
- IgG2b, kappa
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anti-POU Class 2 Associating Factor 1 (POU2AF1) (AA 148-255) antibody
POU2AF1 适用: 人 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal BOB1-2424 unconjugated
anti-POU Class 2 Associating Factor 1 (POU2AF1) (AA 148-255) antibodyPOU2AF1 适用: 人 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal BOB1-2421 unconjugated
anti-POU Class 2 Associating Factor 1 (POU2AF1) antibodyPOU2AF1 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 4A5 unconjugated Recombinant Antibody
anti-POU Class 2 Associating Factor 1 (POU2AF1) (C-Term) antibodyVerified POU2AF1 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-POU Class 2 Associating Factor 1 (POU2AF1) (AA 1-27), (N-Term) antibodyPOU2AF1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22378 unconjugated
anti-POU Class 2 Associating Factor 1 (POU2AF1) antibodyPOU2AF1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-POU Class 2 Associating Factor 1 (POU2AF1) antibodyPOU2AF1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB1715 unconjugated Recombinant Antibody
anti-POU Class 2 Associating Factor 1 (POU2AF1) antibodyPOU2AF1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB13345 unconjugated
anti-POU Class 2 Associating Factor 1 (POU2AF1) (C-Term) antibodyVerified POU2AF1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-POU Class 2 Associating Factor 1 (POU2AF1) (C-Term) antibodyPOU2AF1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Ramos cells. Spleen or Tonsil
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
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别名
- BOB.1
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背景
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Synonyms: BOB-1, B-cell-specific coactivator OBF-1, OCT-binding factor 1, POU domain class 2-associating factor 1
Gene Symbol: POU2AF1
Tissue Expression: B-cells
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分子量
- 35 kDa
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基因ID
- 5450
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UniProt
- Q16633
抗原
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