MUC5AC 抗体 (CF®594)
Quick Overview for MUC5AC 抗体 (CF®594) (ABIN7657453)
抗原
See all MUC5AC 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- MUC5AC (Mucin 5AC / Gastric Mucin)(2-11M1), CF594 conjugate
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产品特性
- This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the N-terminal cysteine rich part of the peptide core of MUC5AC, which is heavily glycosylated. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. MAb 2-11M1 reacts with the protein backbone exclusively, it only reacts with fully deglycosylated MUC5AC. Therefore, the material under test should also be fully deglycosylated. This can be achieved with standard periodate oxidation method. The success of the deglycosylation can be checked with routine PAS (Periodic Acid Shiff) staining. After deglycosylation, the preparation should no longer be stainable with PAS reagent. Only then 2-11M1 will react should MUC5AC be present. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
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免疫原
- M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
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亚型
- IgG1, kappa
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anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 5528-5627) antibody
MUC5AC 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 8H8G6 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) antibodyMUC5AC 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 28-327) antibodyMUC5AC 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 6C9D11 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) antibodyMUC5AC 适用: 人, 小鼠, 大鼠, 猴, Cat, 兔, 小鸡, Pig, Hedgehog IHC, StM 宿主: 小鼠 Monoclonal 45M1 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 5528-5627) antibodyMUC5AC 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 7E9D8 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 601-700) antibodyMUC5AC 适用: 人, 小鼠, 大鼠 ELISA, ICC, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 430-549) antibodyMUC5AC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 351-450) antibodyMUC5AC 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H7 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) antibodyMUC5AC 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-109M unconjugated Recombinant Antibody
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (C-Term) antibodyMUC5AC 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA: Use at 2-4 μg/mL for coating. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Western blot: 1-2 μg/mL. Flow cytometry: 0.5-1 μg/million cells. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: MCF-7 cells. Human colon or stomach (IHC).
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))
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别名
- MUC5AC
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背景
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Synonyms: Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)
Gene Symbol: MUC5AC
Tissue Expression: Epithelial cells
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分子量
- >1,000 kDa
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基因ID
- 4586
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UniProt
- P98088
抗原
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