Cyclin B2 抗体 (CF®488A)
Quick Overview for Cyclin B2 抗体 (CF®488A) (ABIN7652748)
抗原
See all Cyclin B2 (CCNB2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Cyclin B2(X29.2), CF488A conjugate
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产品特性
- In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length Xenopus laevis Cyclin B2 protein
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亚型
- IgG1, kappa
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anti-Cyclin B2 (CCNB2) antibody
CCNB2 适用: 人, 小鼠, 大鼠 WB, IF (cc), IHC (p) 宿主: 兔 Monoclonal 2F4 unconjugated
anti-Cyclin B2 (CCNB2) (AA 1-100) antibodyCCNB2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B2 (CCNB2) (AA 58-398) antibodyCCNB2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B2 (CCNB2) (AA 7-163) antibodyCCNB2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B2 (CCNB2) (Internal Region) antibodyCCNB2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B2 (CCNB2) antibodyCCNB2 适用: 人, 小鼠, 大鼠, 非洲爪蟾 ELISA, IHC, Coat 宿主: 小鼠 Monoclonal X29-2 unconjugated
anti-Cyclin B2 (CCNB2) (AA 1-100) antibodyCCNB2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B2 (CCNB2) antibodyCCNB2 适用: 人 DB 宿主: 小鼠 Monoclonal 2115C1 unconjugated
anti-Cyclin B2 (CCNB2) (pThr359) antibodyCCNB2 适用: 人 DB 宿主: 兔 Polyclonal RB42151 unconjugated
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应用备注
- ELISA: For coating, order antibody without BSA, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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说明
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Positive Control: HeLa cells. Human cervical tissue (IHC).
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Cyclin B2 (CCNB2)
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别名
- Cyclin B2
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背景
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Synonyms: ccnb2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, HsT17299, MGC108931, MGC140694
Gene Symbol: CCNB2
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分子量
- 51 kDa
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基因ID
- 9133
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UniProt
- O95067
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途径
- Cell Division Cycle, M Phase
抗原
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