CDC20 抗体 (His6) (CF®740)
Quick Overview for CDC20 抗体 (His6) (CF®740) (ABIN7652356)
抗原
See all CDC20 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- His6
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原理
- Cdc20(AR12), CF740 conjugate
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产品特性
- Cyclins, regulatory subunits that associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Urea-denatured His6 Cdc20 human recombinant protein
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亚型
- IgG1, kappa
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anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-270) antibody
CDC20 适用: 人 WB, IF, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) antibodyCDC20 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-270) antibodyCDC20 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 57-84) antibodyCDC20 适用: 人 WB, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) antibodyCDC20 适用: 人 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal AR12 unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠, Pig, 马, 豚鼠, 猴, Cow, 非洲爪蟾, Bat, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-499) antibodyCDC20 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 57-84), (N-Term) antibodyCDC20 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB23122 unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠, Pig, 犬, 马, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Ramos or HeLa cells. Tonsil or gastric carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
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别名
- Cdc20
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背景
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Synonyms: CDC20, CDC20A, p55CDC, P55CDC-LSB
Gene Symbol: CDC20
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分子量
- 55 kDa
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基因ID
- 991
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UniProt
- Q12834
抗原
-
