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Recombinant CEA 抗体 (CF®640R)

This anti-CEA antibody is a 兔 单克隆 antibody detecting CEA in IHC 和 IHC (fp). Suitable for 人.
产品编号 ABIN7650551
发货至: 中国

Quick Overview for Recombinant CEA 抗体 (CF®640R) (ABIN7650551)

抗原

See all CEA 抗体
CEA (Carcinoembryonic Antigen Gene Family (CEA))

抗体类型

Recombinant Antibody

适用

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克隆类型

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单克隆

标记

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This CEA antibody is conjugated to CF®640R

应用范围

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Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

C66-2055R
  • 原理

    Carcinoembryonic Antigen (CEA) / CD66 (C66/2055R), CF640R conjugate

    产品特性

    This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA ) from pleural mesotheliomas (rarely or weakly CEA ). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant full-length human CEA protein

    亚型

    IgG
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: MCF7 or 293T cells. Colon carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % BSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    CEA (Carcinoembryonic Antigen Gene Family (CEA))

    别名

    Carcinoembryonic Antigen

    背景

    Synonyms: Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)

    Gene Symbol: CEACAM1 & CEACAM5

    分子量

    80-200 kDa

    基因ID

    1048, 634

    UniProt

    P06731
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