GFP 抗体 (AA 25-120) (FITC)
Quick Overview for GFP 抗体 (AA 25-120) (FITC) (ABIN729440)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 25-120
-
交叉反应
- Pig, 标记
-
交叉反应 (详细)
- Aequorea victoria, Aequorea coerulescens (belt jellyfish)
-
纯化方法
- Purified by Protein A.
-
免疫原
- KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein
-
亚型
- IgG
-
-
-
-
应用备注
-
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 μg/μL
-
缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
-
储存液
- ProClin
-
注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
-
有效期
- 12 months
-
-
- GFP (Green Fluorescent Protein (GFP))
-
别名
- GFP
-
背景
-
Synonyms: Green fluorescence protein, GRP, GFP TAG, GFP-TAG.
Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
-
UniProt
- P42212
抗原
-