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GFP 抗体 (AA 25-120)

This anti-GFP antibody is a 兔 多克隆 antibody detecting GFP in WB, ELISA, IHC (fro), IHC (p), IF (p) 和 IF (cc). Suitable for Aequorea victoria 和 Aequorea coerulescens (Belt Jellyfish).
产品编号 ABIN729433
发货至: 中国

Quick Overview for GFP 抗体 (AA 25-120) (ABIN729433)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

适用

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Aequorea victoria, Aequorea coerulescens (Belt Jellyfish)

宿主

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克隆类型

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多克隆

标记

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This GFP antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
  • 抗原表位

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    AA 25-120

    交叉反应

    Pig, 标记

    交叉反应 (详细)

    Aequorea victoria, Aequorea coerulescens (belt jellyfish)

    纯化方法

    Purified by Protein A.

    免疫原

    KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein

    亚型

    IgG
  • 应用备注

    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 μg/μL

    缓冲液

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    有效期

    12 months
  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP

    背景

    Synonyms: Green fluorescence protein, GRP, GFP TAG, GFP-TAG.

    Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

    UniProt

    P42212
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