YBX1 抗体 (Middle Region)
Quick Overview for YBX1 抗体 (Middle Region) (ABIN7603105)
抗原
See all YBX1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-YB1/Ybx1 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-YB1/Ybx1 Antibody Picoband® (ABIN7603105). Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of mouse YB1/Ybx1, identical to the related rat sequences.
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
1. Bhullar, J., Sollars, V. E. YBX1 expression and function in early hematopoiesis and leukemic cells. Immunogenetics 63: 337-350, 2011. 2. Coles, L. S., Diamond, P., Occhiodoro, F., Vadas, M. A., Shannon, M. F. Cold shock domain proteins repress transcription from the GM-CSF promoter. Nucleic Acids Res. 24: 2311-2317, 1996. 3. Didier, D. K., Schiffenbauer, J., Woulfe, S. L., Zacheis, M., Schwartz, B. D. Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box. Proc. Nat. Acad. Sci. 85: 7322-7326, 1988. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- YBX1 (Y Box Binding Protein 1 (YBX1))
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别名
- Ybx1
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背景
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Synonyms: Neuronal migration protein doublecortin,Doublin,Lissencephalin-X,Lis-X,DCX,DBCN, LISX,
Tissue Specificity: Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
Background: Y box binding protein 1 also known as Y-box transcription factor or nuclease-sensitive element-binding protein 1 is a protein that in humans is encoded by the YBX1 gene. Enables chromatin binding activity, mRNA binding activity, and single-stranded DNA binding activity. Involved in epidermis development and negative regulation of cellular senescence. Acts upstream of or within several processes, including cellular response to interleukin-7, negative regulation of striated muscle cell differentiation, and negative regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Part of histone pre-mRNA 3'end processing complex. Is expressed in several structures, including branchial arch, central nervous system, facial prominence, gonad, and retina. Orthologous to human YBX1 (Y-box binding protein 1).
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分子量
- 50 kDa
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基因ID
- 22608
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UniProt
- P62960
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途径
- Regulation of Muscle Cell Differentiation
抗原
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