BNIP3L/NIX 抗体 (Middle Region)
Quick Overview for BNIP3L/NIX 抗体 (Middle Region) (ABIN7603024)
抗原
See all BNIP3L/NIX (BNIP3L) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-BNIP3L Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins
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产品特性
- Anti-BNIP3L Antibody Picoband® (ABIN7603024). Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human BNIP3L, identical to the related mouse sequences.
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Chen, G., Cizeau, J., Vande Velde, C., Park, J. H., Bozek, G., Bolton, J., Shi, L., Dubik, D., Greenberg, A. Nix and nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J. Biol. Chem. 274: 7-10, 1999. 2. Gao, F., Chen, D., Si, J., Hu, Q., Qin, Z., Fang, M., Wang, G. The mitochondrial protein BNIP3L is the substrate of PARK2 and mediates mitophagy in PINK1/PARK2 pathway. Hum. Molec. Genet. 24: 2528-2538, 2015. 3. Matsushima, M., Fujiwara, T., Takahashi, E., Minaguchi, T., Eguchi, Y., Tsujimoto, Y., Suzumori, K., Nakamura, Y. Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 21: 230-235, 1998. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))
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别名
- BNIP3L
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背景
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Synonyms: BNIP3L, BNIP3A, BNIP3H, NIX, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, NIP3-like protein X, NIP3L
Background: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like is a protein that in humans is encoded by the BNIP3L gene. This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c.
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分子量
- 35 kDa
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基因ID
- 665
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UniProt
- O60238
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途径
- Autophagy
抗原
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