ASCL1 抗体 (C-Term)
Quick Overview for ASCL1 抗体 (C-Term) (ABIN7602851)
抗原
See all ASCL1 抗体适用
宿主
克隆类型
标记
应用范围
- 
    
- 
                                            
抗原表位
 - C-Term
 - 
                                            
原理
 - Anti-ASCL1 Antibody Picoband®
 - 
                                            
交叉反应 (详细)
 - No cross-reactivity with other proteins
 - 
                                            
产品特性
 - Anti-ASCL1 Antibody Picoband® (ABIN7602851). Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
 - 
                                            
纯化方法
 - Immunogen affinity purified.
 - 
                                            
免疫原
 - A synthetic peptide corresponding to a sequence at the C-terminus of human ASCL1, identical to the related mouse and rat sequences.
 - 
                                            
亚型
 - IgG
 
 - 
                                            
 - 
    
 - 
    
- 
                                            
应用备注
 - 
                        Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Ahmad, I. Mash-1 is expressed during ROD photoreceptor differentiation and binds an E-box, E(opsin-1), in the rat opsin gene. Brain Res. Dev. Brain Res. 90: 184-189, 1995. 2. Ball, D. W., Azzoli, C. G., Baylin, S. B., Chi, D., Dou, S., Donis-Keller, H., Cumaraswamy, A., Borges, M., Nelkin, B. D. Identification of a human achaete-scute homolog highly expressed in neuroendocrine tumors. Proc. Nat. Acad. Sci. 90: 5648-5652, 1993. 3. Caiazzo, M., Dell'Anno, M. T., Dvoretskova, E., Lazarevic, D., Taverna, S., Leo, D., Sotnikova, T. D., Menegon, A., Roncaglia, P., Colciago, G., Russo, G., Carninci, P., Pezzoli, G., Gainetdinov, R. R., Gustincich, S., Dityatev, A., Broccoli, V. Direct generation of functional dopaminergic neurons from mouse and human fibroblasts. Nature 476: 224-227, 2011. - 
                                            
限制
 - 仅限研究用
 
 - 
                                            
 - 
    
- 
                                            
状态
 - Lyophilized
 - 
                                            
溶解方式
 - Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
 - 
                                            
浓度
 - 500 μg/mL
 - 
                                            
缓冲液
 - Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
 - 
                                            
储存条件
 - 4 °C,-20 °C
 - 
                                            
储存方法
 - 
                        At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. 
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. 
 - 
                                            
 - 
    
- ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))
 - 
                                            
别名
 - ASCL1
 - 
                                            
背景
 - 
                        
Synonyms: ASCL1, ASH1, BHLHA46, HASH1, Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46
Background: Achaete-scute homolog 1 is a protein that in humans is encoded by the ASCL1 gene. This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.
 - 
                                            
分子量
 - 30 kDa
 - 
                                            
基因ID
 - 429
 - 
                                            
UniProt
 - P50553
 - 
                                            
途径
 - Dopaminergic Neurogenesis
 
抗原
 -